Inzira zinyuranye (LFAs) ziroroshye gukoresha, ibikoresho byo kwisuzumisha bishobora gupima biomarkers mubitegererezo nka macandwe, amaraso, inkari, nibiryo.Ibizamini bifite inyungu nyinshi kurenza ubundi buhanga bwo gusuzuma harimo:
Ubworoherane: Ubworoherane bwo gukoresha ibi bizamini ntaho bihuriye - kongeramo ibitonyanga bike kurugero rwicyitegererezo hanyuma usome ibisubizo byawe mumaso nyuma yiminota mike.
Ubukungu: Ibizamini ntibihendutse - mubisanzwe munsi yidolari rimwe kuri buri kizamini cyo gukora mubipimo.
Gukomera: Ibizamini birashobora kubikwa ku bushyuhe bw’ibidukikije kandi bikagira ubuzima bwimyaka myinshi.
Buri mwaka hakorwa amamiliyaridi y’ibizamini kugira ngo hamenyekane indwara zandurira mu mibonano mpuzabitsina, indwara ziterwa n’umubu, igituntu, hepatite, gutwita no gupima uburumbuke, ibimenyetso byumutima, gupima cholesterol / lipide, ibiyobyabwenge byo guhohotera, gusuzuma amatungo, hamwe n’umutekano w’ibiribwa, muri byo abandi.
LFA igizwe nicyitegererezo cyicyitegererezo, padi ya conjugate, umurongo wa nitrocellulose urimo imirongo yikizamini no kugenzura, hamwe na padi.Buri kintu cyose cyuzuzanya byibura mm2-22 ituma capillary itambuka yintangarugero.
Kugira ngo ukoreshe igikoresho, icyitegererezo cyamazi nkamaraso, serumu, plasma, inkari, amacandwe, cyangwa ibishishwa bya solubilised, byongewe kumurongo wikitegererezo kandi ni bibi binyuze mubikoresho bitembera.Icyitegererezo cyerekana intangarugero kandi ikayungurura uduce tutifuzwa nka selile zitukura.Icyitegererezo kirashobora gutemba ntakabuza kuri padi ya conjugate irimo amabara akomeye cyangwa florescent nanoparticles ifite antibody hejuru yabo.Iyo isukari igeze kuri padi ya conjugate, iyi nanoparticles yumye irekurwa ikavangwa nicyitegererezo.Niba hari intego zisesenguye murugero antibody imenya, izihuza na antibody.Nanoparticles ihujwe na analyte noneho inyura muri nitrocellulose ya membrane no kuruhande rumwe cyangwa nyinshi zipimisha n'umurongo wo kugenzura.Umurongo wikizamini (wanditseho T mumashusho hejuru) nicyo kintu cyambere gisomwa cyo kwisuzumisha kandi kigizwe na poroteyine zidafite imbaraga zishobora guhuza nanoparticle kugirango habeho ikimenyetso gifitanye isano na analyte muri sample.Amazi akomeza gutembera kumurongo kugeza ageze kumurongo ugenzura.Umurongo wo kugenzura (wanditseho C mumashusho hejuru) urimo ligande ya affinity izahuza nanoparticle conjugate cyangwa idafite analyte ihari mugisubizo kugirango hemezwe ko assay ikora neza.Nyuma yumurongo wigenzura, amazi atembera mumashanyarazi akenewe kugirango yinjize ibintu byose byamazi kugirango harebwe niba hari urujya n'uruza rw'ibizamini no kugenzura.Mubigeragezo bimwe na bimwe, kwirukana buffer byashyizwe kumurongo wicyitegererezo nyuma yicyitegererezo kugirango tumenye neza ko ibyitegererezo byose bitwarwa kumurongo.Icyitegererezo cyose kimaze kurenga ikizamini no kugenzura imirongo, assay iruzuye kandi uyikoresha arashobora gusoma ibisubizo.
Igihe cyo gusesengura giterwa n'ubwoko bwa membrane ikoreshwa muburyo bwo gutembera (ibinini binini bitemba vuba ariko muri rusange ntibyoroshye) kandi mubisanzwe byuzuye muminota itarenze 15.
Igihe cyo kohereza: Ugushyingo-27-2021